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Journal of Chromatography B: Biomedical Sciences and Applications
Copyright © 2001 Elsevier Science B.V. All rights reserved

Volume 737, Issues 1-2,
Pages 1-305 (14 January 2000)

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1.
Foreword, Page 1
A. Author
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2.
Two-step purification method of vitellogenin from three teleost fish species: rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus), Pages 3-12
François Brion, Françoise Rogerieux, Patrice Noury, Bernard Migeon, Patrick Flammarion, Eric Thybaud and Jean-Marc Porcher
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3.
Two-step fast protein liquid chromatographic purification of the Serratia marcescens hemolysin and peptide mapping with mass spectrometry, Pages 13-23
Ralf Hertle, Roderich Süssmuth, Volkmar Braun and Günther Jung
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4.
Preparative two-step purification of recombinant human basic fibroblast growth factor from high-cell-density cultivation of Eschericia coli, Pages 25-38
Gunnar Garke, Wolf-Dieter Deckwer and Friedrich Birger Anspach
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5.
Purification of the Ca2+-binding protein S100A1 from myocardium and recombinant Escherichia coli, Pages 39-45
Philipp Ehlermann, Andrew Remppis, Patrick Most, Juliane Bernotat, Claus W. Heizmann and Hugo A. Katus
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6.
Multi-step purification strategy for RANTES wild-type and mutated analogues expressed in a baculovirus system, Pages 47-54
Barbara Comuzzi, Cinzia Arcelloni, Simona Polo, Vanessa Nardese, Paolo Lusso and Rita Paroni
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7.
Purification of the c-erbB2/neu membrane-spanning segment:; a hydrophobic challenge, Pages 55-61
M. Goetz, F. Rusconi, M. Belghazi, J. M. Schmitter and E. J. Dufourc
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8.
Chromatographic purification of the CH2 domain of the monoclonal antibody MAK33, Pages 63-69
Michael J. W. Thies and Franziska Pirkl
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9.
Three-step purification of a fragment of the large immunophilin FKBP52, Pages 71-76
Franziska Pirkl
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10.
Purification of a viral coat protein by an engineered polyionic sequence, Pages 77-84
K. Stubenrauch, A. Bachmann, R. Rudolph and H. Lilie
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11.
Expression, purification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein, Pages 85-95
Béatrice Tallet, Thérèse Astier-Gin, Danielle Londos-Gagliardi and Bernard Guillemain
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12.
Purification of respiratory syncytial virus F and G proteins, Pages 97-106
Christian Roder, Torsten Krusat, Kerstin Reimers and Hermann Werchau
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13.
Three-step purification of bacterially expressed human single-chain Fv antibodies for clinical applications, Pages 107-117
J. Laroche-Traineau, G. Clofent-Sanchez and X. Santarelli
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14.
Purification of the membrane binding domain of cytochrome b5 by immobilised nickel chelate chromatography, Pages 119-130
Rukhsana R. Begum, Richard J. Newbold and David Whitford
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15.
Novel, rapid purification of the membrane protein photosystem I by high-performance liquid chromatography on porous materials, Pages 131-142
Stephan-Olav Wenk and Jochen Kruip
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16.
Three-step chromatographic purification procedure for the production of a His-tag recombinant kinesin overexpressed in E. coli, Pages 143-150
S. Gibert, N. Bakalara and X. Santarelli
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17.
Improved purification of the membrane-bound hydrogenase¯sulfur-reductase complex from thermophilic archaea using -aminocaproic acid-containing chromatography buffers, Pages 151-160
Simone Laska and Arnulf Kletzin
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18.
Highly efficient purification of porcine diamine oxidase, Pages 161-166
Doris Wilflingseder and Hubert G. Schwelberger
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19.
Development of a downstream process for the isolation of Staphylococcus aureus arsenate reductase overproduced in Escherichia coli, Pages 167-178
Joris Messens, Gaynor Hayburn, Elke Brosens, Georges Laus and Lode Wyns
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20.
Purification of recombinant hydantoinase and -N-carbamoylase from Arthrobacter aurescens expressed in Escherichia coli: comparison of wild-type and genetically modified proteins, Pages 179-186
Markus Pietzsch, Anja Wiese, Kerstin Ragnitz, Burkhard Wilms, Josef Altenbuchner, Ralf Mattes and Christoph Syldatk
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21.
Purification of a -hydantoinase using a laboratory-scale Streamline phenyl column as the initial step, Pages 187-194
Jan Abendroth, Shivani Chatterjee and Dietmar Schomburg
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22.
-Xylose metabolism by Candida intermedia: isolation and characterisation of two forms of aldose reductase with different coenzyme specificities, Pages 195-202
Peter Mayr, Kaspar Brüggler, Klaus D. Kulbe and Bernd Nidetzky
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23.
Purification of surface-associated urease from Helicobacter pylori, Pages 203-212
Elmar Rokita, Athanasios Makristathis, Alexander M. Hirschl and Manfred L. Rotter
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24.
Expression of chymotrypsin(ogen) in the thioredoxin reductase deficient mutant strain of Escherichia coli AD494(DE3) and purification via a fusion product with a hexahistidine-tail, Pages 213-224
Gert Verheyden, Guido Volckaert and Yves Engelborghs
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25.
Refolding and purification of a urokinase plasminogen activator fragment by chromatography, Pages 225-235
Edward M. Fahey, Julian B. Chaudhuri and Peter Binding
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26.
Purification of human adenosine deaminase for the preparation of a reference material, Pages 237-244
Alexandre Bota, F. -Javier Gella and Francesca Canalias
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27.
Pullulanase from the hyperthermophilic bacterium Thermotoga maritima: purification by -cyclodextrin affinity chromatography, Pages 245-251
Gernot Kriegshäuser and Wolfgang Liebl
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28.
Single-step purification of a recombinant thermostable -amylase after solubilization of the enzyme from insoluble aggregates, Pages 253-259
Anni Linden, Frank Niehaus and Garabed Antranikian
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29.
Technique for a rapid and efficient purification of the SHV-1 and PSE-2 -lactamases, Pages 261-265
Fabrice Bouillenne, André Matagne, Bernard Joris and Jean-Marie Frère
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30.
Purification of the fengycin synthetase multienzyme system from Bacillus subtilis b213, Pages 267-275
Sigrid Steller and Joachim Vater
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31.
New approach for separating Bacillus subtilis metalloprotease and -amylase by affinity chromatography and for purifying neutral protease by hydrophobic chromatography, Pages 277-284
Isabelle Lauer, Béatrice Bonnewitz, Agnès Meunier and Marc Beverini
SummaryPlus | Article | Journal Format-PDF (343 K)
32.
Purification and characterization of an aminopeptidase from the chloroplast stroma of barley leaves by chromatographic and electrophoretic methods, Pages 285-293
Marcelo Desimone, Martina Krüger, Tim Wessel, Marco Wehofsky, Ralf Hoffmann and Edgar Wagner
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33.
Three-step chromatographic purification of Cpr6, a cyclophilin from Saccharomyces cerevisiae, Pages 295-299
Christian Mayr
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34.
Index, Pages 301-302

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35.
Index, Pages 303-305

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